Using the term "MFI", it is a good idea to clearly define it in your context. It really depends on how your cells are expressing the markers that you use. There are many reasons to choose a mean over a median over a mode, or vice versa. Note- A common question is "Which statistic should I use?". of Grandparent - The percentage of events (cells) in this gate out of the gate two levels up.Ĭount - The absolute number of events (cells) in a gate. of Parent - The percentage of events (cells) in this gate out of the parent gate (one level up).įreq. n=50 is equivalent to the median.įrequency - The percentage of events (cells) in this gate out of the total number of events in the sample.įreq. This is the relative intensity below which n% of the events are found, where n is the selected value. The robust CV is not as skewed by outlying values as the CV. Robust CV - Equals 100 * 1/2( Intensity - Intensity ) / Median. 1/CV is a common way to define the Signal to Noise Ratio. In FlowJo, the CV statistic is displayed in percent (i.e. Lower values indicate the data points are closer to the mean and give higher confidence to the mean value.ĬV - The Coefficient of Variation is a normalized Standard Deviation. refer to the specific sections of this website or contact the FCF staff.
Flowjo reference software#
SD - The Standard Deviation is a measure of the spread of the dataset. FlowJo is the main flow cytometry data analysis software recommended by the FCF. Less than or equal to the arithmetic mean. Can be a more applicable metric for a log-normal distribution. This is the same intensity value at which the highest point on a histogram is found. Mode - The relative intensity value which is most frequently found for a given parameter. In general, the median is a more robust estimator of the central tendency of a population than the mean. Median - The median is the relative intensity value below which 50% of the events are found i.e., it is the 50th percentile. For a normal distribution, the mean = median = mode. If you use the Calibration Platform to calibrate a fluorescence parameter to absolute units, then all statistics computed on the calibrated platform are given in terms of those absolute units. To get the options for channel statistics, hold down the option key while clicking the statistic button Use an integer scale for relative intensity ranging from 0 to the maximum value defined by the data collection program (i.e 255, 1023, 4095). Channel numbers ignore the scaling factors, and The scale values take into account scaling factors such as the gain setting for the parameter. Relative intensity values are in arbitrary units and can be expressed as scale values or channel numbers. FlowJo is currently developed by the Ashland, Oregon-based FlowJo LLC, a subsidiary of Becton Dickinson.This page details the statistics that can be selected from the Statistics Dialog window and appear in both the workspace and the graph window.
Flowjo reference windows#
In 2002, Tree Star released a Windows version. calcium flux analysis, proliferation analysis, quantification, cluster identification and backgating displayįlowJo became a commercial product in 1996. We then evaluated the performance of three reference-based in-silico cell mixture deconvolution methods: Houseman et al.’s method, IDOL, and EPIC IDOL-Ext 18, 29, 32. Thus, you can apply a gate to a sample, copy it to the group, and that gate will be automatically placed on all samples in the group.įlowJo provides tools for the creation of histogram and other plot overlays, cell cycle analysis. When an operation on a group is initiated, FlowJo can perform the same operation on every sample belonging to that group.
Within a workspace, samples can be grouped or sorted by various attributes such as the panel of antibodies with which they are stained, tissue type, or patient from whom they came.
FlowJo's ability to automate repetitive operations facilitates the production of statistics tables and graphical reports when the experiment involves many samples, parameters and/or operations. Viewing an entire experiment in a Workspace permits organizing and managing complex cytometry experiments and produces detailed graphical reports. In FlowJo, samples are organized in a "Workspace" window, which presents a hierarchical view of all the samples and their analyses (gates and statistics).
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